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Genome Rearrangement and Nitrogen Fixation in Anabaena Blocked by Inactivation of xisA Gene
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New Series, Vol. 242, No. 4884 (Dec. 9, 1988), pp. 1421-1423 (3 pages)
Published By: American Association for the Advancement of Science
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Abstract

Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of an Anabaena photosynthetic vegetative cell into a nitrogen-fixing heterocyst. The xisA gene, located on the nifD 11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of the xisA gene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of the Anabaena xisA gene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation.

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New Series, Vol. 242, No. 4884 (Dec. 9, 1988), pp. 1421-1423 (3 pages)
Published By: American Association for the Advancement of Science
Cite this Item

Copy Citation

Export Citation

Export a RIS file (For EndNote, ProCite, Reference Manager, Zotero, Mendeley…)
Note: Always review your references and make any necessary corrections before using. Pay attention to names, capitalization, and dates.
Abstract

Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of an Anabaena photosynthetic vegetative cell into a nitrogen-fixing heterocyst. The xisA gene, located on the nifD 11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of the xisA gene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of the Anabaena xisA gene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation.

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